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Stefanou J, Agelopoulou AV, Sipsas NV, Smilakou N, Avlami A. Moraxella catarrhalis endocarditis: case report and review of the literature. . 2000. 32(2):217-8. .

Narinesingh SP, Whitby DJ, Davenport PJ. Moraxella catarrhalis: an unrecognized pathogen of the oral cavity?. . 2011 Jul. 48(4):462-4. .

Tritton D, Watts T, Sieratzki JS. Peri-orbital cellulitis and sepsis by Branhamella catarrhalis. . 1998 Jul. 157(7):611-2. .

Abdolrasouli A, Amin A, Baharsefat M, Roushan A, Hemmati Y. Moraxella catarrhalis associated with acute urethritis imitating gonorrhoea acquired by oral-genital contact. . 2007 Aug. 18(8):579-80. .

Uehara Y, Yagoshi M, Tanimichi Y, Yamada H, Shimoguchi K, Yamamoto S, et al. Impact of reporting gram stain results from blood culture bottles on the selection of antimicrobial agents. . 2009 Jul. 132(1):18-25. . .

Hwang PH. A 51-year-old woman with acute onset of facial pressure, rhinorrhea, and tooth pain: review of acute rhinosinusitis. . 2009 May 6. 301(17):1798-807. .

Roland PS, Kreisler LS, Reese B, et al. Topical ciprofloxacin/dexamethasone otic suspension is superior to ofloxacin otic solution in the treatment of children with acute otitis media with otorrhea through tympanostomy tubes. . 2004 Jan. 113(1 Pt 1):e40-6. . .

Swanson RN, Lainez-Ventosilla A, De Salvo MC, Dunne MW, Amsden GW. Once-daily azithromycin for 3 days compared with clarithromycin for 10 days for acute exacerbation of chronic bronchitis: a multicenter, double-blind, randomized study. . 2005. 4(1):31-9. .

Sethi S, Anzueto A, Farrell DJ. Antibiotic activity of telithromycin and comparators against bacterial pathogens isolated from 3,043 patients with acute exacerbation of chronic bronchitis. . 2005 Mar 8. 4:5. . .

Dore DD, DiBello JR, Lapane KL. Telithromycin use and spontaneous reports of hepatotoxicity. . 2007. 30(8):697-703. .

Yoshida K, Okimoto N, Kishimoto M, et al. Efficacy and safety of moxifloxacin for community-acquired bacterial pneumonia based on pharmacokinetic analysis. . 2011 Oct. 17(5):678-85. .

Ren D, Yu S, Gao S, et al. Mutant lipooligosaccharide-based conjugate vaccine demonstrates a broad-spectrum effectiveness against Moraxella catarrhalis. . 2011 Jun 6. 29(25):4210-7. . .

Yang M, Johnson A, Murphy TF. Characterization and evaluation of the Moraxella catarrhalis oligopeptide permease A as a mucosal vaccine antigen. . 2011 Feb. 79(2):846-57. . .

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Michael Constantinescu, MD Staff Pathologist, Overton Brooks Veterans Affairs Medical Center Michael Constantinescu, MD is a member of the following medical societies: American Society for Clinical Pathology , Petit New Standard jeans Blue APC Buy Online New Order Cheap Online wCZQnf8C
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Until very recently, this was undoubtedly one of the most sought-after Alexa integrations, spawning numerous IFTTT integrations.

For the past few months, you could only control the Next Thermostat through IFTTT or the Wink hub, but now it has been integrated directly with Alexa.

Not one to let startups steal its thunder, long-time thermostat maker Honeywell created its own take on the Nest concept called the Honeywell Lyric Thermostat.

The latest version works with Apple HomeKit and now Alexa, thanks to the company's integration with SmartThings.

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While not as pretty as their cousin, the Lyric thermostat, Honeywell Total Connect Comfort thermostats are just as feature rich, but with a bigger focus on scheduling. And thanks to an official integration with SmartThings, they can now be controlled by Alexa as well.

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was the first smart thermostat to integrate directly with Alexa, without the need for a hub. It was also one of the first thermostats to support Apple HomeKit.

This April, Alexa will be able to control Haiku Home ceiling fans, with the ability to adjust the lights and fan speed. All Haiku fans with the company's SenseMe technology, including its H, I, and L Series fans, will work with this new integration.

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The Echo and Alexa are quickly conquering the home, and with new integrations like Ford Sync they're also popping up in our cars and garages. There aren't many compatible devices on the market just yet, but it's only a matter of time until more are released.

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is a small device that connects to your car's diagnostic port and beams driving data like fuel level, check engine light codes, and more to your smartphone via Bluetooth.

With the company's Alexa integration , you can ask Alexa how much fuel you have, where you last parked, and even how much you drove over a given time period.

Garageio is a small, WiFi-enabled device that you can connect to up to three garage doors (depending on the model you buy) to remotely open and close them from your phone. With the Amazon Echo and similar devices, you can open and close your garage doors simply by Ines sandwashedsilk pyjama set Morpho Luna Low Price Fee Shipping Cheap Price Eotwg0q
asking Alexa.

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Accompanying the description of a shuttle vector for C. trachomatis by Wang et al. ( 26 ) were two studies reporting the use of ethyl methanesulfonate (EMS) (an alkylating agent that causes G-to-A transition mutations) for chemical mutagenesis of C. trachomatis . In the first study, Kari et al. used low levels of EMS targeting a mutation rate of one base per genome to generate a library of C. trachomatis mutants ( Footlocker Cheap Price Womens Alley Dress Fashion Union Clearance Ebay Sale Outlet Sale Authentic VDd81lw
). The library strains were then pooled and assessed for the desired mutation using PCR, followed by CELI digestion. This method is known as t argeting- i nduced l ocal l esions in g enome, or TILLING ( Free Shipping Sale Online Pay With Paypal Online Womens D2073/3073 2Piece Swimsuit Haute Pression Buy Cheap Nicekicks Outlet Online Shop gfRFAZTf
). To detect mutants, PCR amplicons from each pool are heat denatured and then allowed to anneal. If a pool contains wild-type and mutant strains, a heteroduplex will form due to a base mismatch, which allows for cleavage by CELI and detection of the cleaved product by DNA gel electrophoresis. The desired clones can then be obtained from the mutant library by PCR screening of clones obtained through limiting dilution or plaque assays followed by sequencing to map the exact mutation. Mutant phenotypes can then be assessed to determine gene function. Once the EMS mutant library pools are established, one can search for all desired mutants within the pool. Limitations of this method include extensive hands-on work to both create the library and identify mutants along with back-end whole-genome sequencing to confirm the clonal isolate contains a mutation only in the gene of interest. This reverse genetic approach has been used to study the function of trpB and pmpD in C. trachomatis and has been adapted for use with C. muridarum ( 28 , 67 , 68 ).

View larger version:
FIG 2

Chemical mutagenesis methods used for TILLING (A) or forward genetic approaches (B) are shown. The green and red lines in the wells in the TILLING section represent wild-type and mutant amplicons, respectively (see the text). Both methods require whole-genome sequencing to map mutations and may require lateral gene transfer and homologous recombination between the mutant (yellow) and parental (green) strains to isolate mutant alleles. Chemical mutants (and intron-generated mutants) can be complemented using shuttle vectors ( Fig. 1 ).

EMS has also proven to be a useful tool for forward genetics in C. trachomatis . Using increased amounts of EMS compared to those used by Kari et al. ( 28 ) to generate 3 to 20 mutations per genome, Nguyen and Valdivia ( Buy Cheap For Cheap Womens Starlet Slim Jeans Guess Exclusive Order Online Online Sale Buy Cheap Best Seller bDC7Ctzxn
) prepared a library of mutants that were then assessed for growth defects via automated analysis of plaque morphology ( Fig. 2B ). Strains giving rise to altered plaques were isolated, expanded in cell culture, and genotyped using whole-genome sequencing. As strains contained multiple mutations, lateral gene transfer and homologous recombination as initially described by Demars et al. ( 37 ) was then used to link gene lesions with specific phenotypes. This high-content mutagenesis approach has been further expanded by Kokes et al. ( 70 ) using either EMS or N -ethyl- N -nitrosourea (ENU) (alkylating agent leading to A-to-T transversion mutation) to mutate C. trachomatis , creating a collection of 934 mutant strains carrying 6 to 25 mutations. Strains were screened via plaque morphology, and whole-genome sequencing was used to map all mutated alleles within the pools of mutant strains. The availability of this sequence data allows mutants of interest to be selected and assessed for their phenotype following segregation of the allele using lateral gene transfer and homologous recombination.

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